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. 2021 May 27;11:11146. doi: 10.1038/s41598-021-89983-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative MSOT images showing the clearance of IRDye800CW from mouse kidneys. IRDye800CW (60 nM dissolved in 0.9% saline) was injected intravenously into WT mice 24 h after sham operation. The clearance/movement of IRDye800CW (green) in the cross section of the right kidney (white square) was recorded. ROIs in cortex and medulla/pelvis region of the kidney are delineated in red and blue, respectively. Representative images of 28 mice (n = 7 in each group). The green bar represents the scale for the mean pixel intensity of the IRDye800CW signal.