Table 1.
Cultivable mucin degraders in EC samples.The viable counts of anaerobic mucinolytic bacteria and Enterobacteriaceae, respectively counted onto plates of MM medium and MacConkey, and the taxonomic attribution of the predominant anaerobic is reported.
| Sample | Anaerobic mucinolytic bacteria Log (cfu/mL) |
Enterobacteriaceae Log (cfu/mL) |
Isolated strains | Ref_seq ID | |
|---|---|---|---|---|---|
| EC 1 | 7.8 ± 0.9 | 7.2 ± 0.5 | Clostridium disporicum | WC0700 | NR_026491.1 |
| Clostridium disporicum | WC0701 | NR_026491.1 | |||
| EC 2 | 8.4 ± 0.7 | 7.3 ± 0.4 | Clostridium disporicum | WC0702 | NR_026491.1 |
| Clostridium disporicum | WC0703 | NR_026491.1 | |||
| EC 3 | 8.5 ± 0.5 | 7.9 ± 0.5 | Clostridium disporicum | WC0704 | NR_026491.1 |
| EC 4 | 8.3 ± 0.6 | 7.6 ± 0.6 | Paraclostridium benzoelyticum | WC0705 | NR_148815.1 |
| Clostridium disporicum | WC0706 | NR_026491.1 | |||
| EC 5 | 8.4 ± 0.5 | 8.0 ± 0.3 | Paraclostridium benzoelyticum | WC0707 | NR_148815.1 |
| Paraclostridium benzoelyticum | WC0708 | NR_148815.1 | |||
| Clostridium tertium | WC0709 | NR_113325.1 | |||
Counts are reported as mean Log (cfu/mL) ± standard deviation, n = 3. For both the media, no significant difference was observed among samples (ANOVA, P > 0.05). Cultivable anaerobic mucinolytic bacteria were significantly more numerous than Enterobacteriaceae (paired samples t-test, P < 0.05). Taxonomic attribution was obtained by alignment of the V1–V4 regions of 16S rRNA gene with the NCBI refseq_rna database using BLAST. The accession number of best hits and the sequence identity (%) obtained with MUSCLE are reported.