Table 2.
Possible tryptic peptides of homicorcin and its variant and their presence in MS analysis.
| Peptide fragment | Domain | Tryptic peptide | m/z | Presence in MS |
|---|---|---|---|---|
| Homicorcin/ homicorcin 1 | ||||
| A/A’ | N-terminal | S(de)LGTAIK/S(hpo)LGTAIK | 653.4/656.8 | + |
| B | N-terminal | AT(de)K | 301.2 | – |
| C | N-terminal | NACPK | 532.2 | − |
| B + C | N-terminal | AT(de)KNACPK | 814.4 | + |
| D | Hinge | VT(de)R | 357.2 | + |
| B + C + D | N-terminal + Hinge | AT(de)KNACPKVT(de)R | 1153.5 | + |
| E | C-terminal | LVT(de)VS(de)CQK | 841.5 | − |
| F | C-terminal | S(de)DCQ | 434.1 | − |
| D + E | Hinge + C-terminal | VT(de)RLVT(de)VS(de)CQK | 1179.7 | − |
| E + F | C-terminal | LVT(de)VS(de)CQKS(de)DCQ | 1256.6 | + |
| D + E + F | Hinge + C-terminal | VT(de)RLVT(de)VS(de)CQKS(de)DCQ | 1594.8 | − |
A’ denotes fragment A of homicorcin 1 where dehydrated first Ser residue was modified to 2-hydroxypropionate (Hpo). Domain name represents the fragment position in the core peptide. Bold and underlined ‘S(de)’ or ‘T(de)’ residues are predicted to be dehydrated and ‘C’ are predicted to form thio-ether linkage with dehydrated S/T residues. . ( +) and (−) represents the presence or absence respectively of the fragment in MS analysis.