Figure 5.

Vitamin D promotes miR-26b-5p expression in placental trophoblasts. (A) 1,25(OH)₂D₃ stimulates miR-26b-5p expression in HTR-8/SVneo cells. The bar graphs show relative miR-26b-5p expression after normalization to U6 expression in each sample from seven independent experiments. *P < 0.05: 100 nM of 1,25(OH)₂D₃ treated vs control. (B) miR-26b-5p expression in HTR-8/SVneo cells treated with CoCl₂ in the presence or absence of 1,25(OH)₂D₃, showing that 1,25(OH)₂D₃ could prevent the CoCl₂-induced decrease in miR-26b-5p expression in placental trophoblasts. The bar graphs show relative miR-26b-5p expression after normalization to U6 expression in each sample from eleven independent experiments. **P < 0.01: 1,25(OH)₂D₃ treated vs control. ^##P < 0.01: CoCl₂ alone vs control. ^###P < 0.001: 1,25(OH)₂D₃ + CoCl₂ vs CoCl₂ alone. (C) Inhibition of VDR expression prevents 1,25(OH)₂D₃-induced increase in miR-26b-5p expression in placental trophoblasts. VDR siRNA was transfected into HTR-8/SVneo cells. The bar graphs show relative miR-26b-5p expression after normalization to U6 in each sample from four independent experiments. *P < 0.05: 1,25(OH)₂D₃ treated vs control. **P < 0.01: VDR siRNA and 1,25(OH)₂D₃ + VDR siRNA vs 1,25(OH)₂D₃ treated alone, respectively.