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. 2021 May 27;11:11210. doi: 10.1038/s41598-021-90700-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-9-5p was significantly associated with PCa cell invasion, migration, and clonal, clonogenic and sphere-forming activities in vitro. From the upper part to the lower part; Transwell migration assays, invasion assays, clonal experiments, 3-D Matrigel clonogenic assays and sphere formation assays were separately performed to determine the metastasis and stemness characteristics of PCa cells. PC3 and DU145 cells were transfected with miR-9-5p mimic, mimic NC, miR-9-5p inhibitor or inhibitor NC for 48 h. All results are shown as the mean ± S.D. from three independent experiments. Statistical analysis was carried out using GraphPad Prism 7.0 Software (GraphPad Software, La Jolla, CA). *P < 0.05, **P < 0.01.