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. 2021 May 27;11:11210. doi: 10.1038/s41598-021-90700-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MiR-9-5P has a promoting role in CD44+ PCSCs. (A) After sorting of CD44+ and CD44− PC3 PCa cells by flow cytometry, decreased expression of miR-9 in the CD44+ PC3 cells was mediated by the transfected miR-9 inhibitor, and the invasion, clone formation and sphere formation abilities of CD44+ PC3 cells were detected 48 h later. (B) Sorting of CD44+ and CD44− PC3 PCa cells was performed by flow cytometry, and high expression of miR-9-5p was detected in the CD44− PC3 cells transfected with the miR-9-5p mimic. After 48 h, the invasion, clone formation and sphere formation abilities of CD44− PC3 cells were detected. The results are represented as the mean ± S.D. from three independent assays. *P < 0.05.