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. 2021 May 27;11:11210. doi: 10.1038/s41598-021-90700-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of miR-9-5p promotes xenograft tumor growth in vivo. (A) Overexpression or decreased expression of miR-9-5p in PC3 or DU145 cells was generated, after which the transfected cells were subcutaneously injected into BALB/C nude mice. Tumors were harvested and the tumor images is shown on the left of the picture. The incidence (tumors/injections) and weight of the tumors (mean ± S.D.) and the corresponding P values are shown on the right of the picture. (B, C) Representative immunohistochemical (IHC) staining pictures are shown, in which the Ki-67- or Caspase-3-positive cells from the harvested tumor tissues were stained (magnification, × 200). The percentage of Ki67-positive or Caspase-3-positive cells was calculated. All results are represented as the mean ± SEM from three independent assays. *P < 0.05, **P < 0.01.