Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 27;11:11167. doi: 10.1038/s41598-021-90653-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Impaired formation of EPI and PrE in embryos derived from single blastomeres. (a–c) 1C, 2CB, 4CB, and 8CB pseudoblastocysts at 96 hpi were cultured for the establishment of ESC lines, which were subjected to immunofluorescence staining with anti-OCT4 and anti-NANOG for determination of their pluripotency. Representative phase-contrast images showing ICM outgrowth of pseudoblastocysts after culture for 14 days are shown in (a). Representative bright-field as well as OCT4 (red) and NANOG (green) immunofluorescence images of established ESC lines are shown in (b). DNA was stained with RedDot2 (white). The rate of ESC line establishment is shown in the left panel of (c), and the difference in the rates among the different types of pseudoblastocyst and its simultaneous 95% CI are shown in the right panel. The P values were determined with the two-sided Fisher’s exact test followed by adjustment with the BH method (FDR = 0.05). **P < 0.001. n indicates the number of pseudoblastocysts. All data were obtained from four replicate experiments. (d,e) 1C, 2CB, 4CB, and 8CB pseudoblastocysts at 120 hpi were stained with anti-GATA4 and anti-DAB2 for determination of the rate of PrE polarization. Representative images showing the expression of GATA4 (red) and apical-side expression of DAB2 (green) in cells located in the PrE compartment of blastocysts are shown in (d). DNA was stained with Hoechst 33342 (blue). The right panels are higher magnification views of the ICM of embryos in the left panels. The rate of PrE polarization is shown in the left panel of (e), and the difference in the rates of PrE polarization among the different types of embryo together with its simultaneous 95% CI are shown in the right panel. The P values were determined as in (c). **P < 0.001. n indicates the number of pseudoblastocysts. All data were obtained from three or four experiments performed on different days for each blastomere stage and were then analyzed. (f) Number of cells in the ICM (left panel) and in TE (right panel) of the blastocysts in Fig. 5e.