Table 1.
List of recently identified 17 circulating KRIS proteins associated with risk of development of ESKD (14) and their corresponding intracellular and extracellular protein levels (expressed as fold changes) following TNFα treatment and hyperglycemia condition.
| Protein name | Gene symbol | Intracellular | Extracellular | ||
|---|---|---|---|---|---|
| Fold change | p value | Fold change | p value | ||
| TNF-RSF members | |||||
| TNF receptor superfamily member 1A | TNFR1 | 1.00 | n.s | 0.37 | 6.2 × 10−6 |
| TNF receptor superfamily member 1B | TNFR2 | 1.06 | n.s | 0.99 | n.s |
| TNF receptor superfamily member 21 | TNFRSF21 | 0.81 | n.s | 0.61 | 2.7 × 10−3 |
| TNF receptor superfamily member 19 | TNFRSF19 | 0.99 | n.s | 0.98 | n.s |
| TNF receptor superfamily member 27 | EDA2R | 1.05 | n.s | 1.10 | n.s |
| TNF receptor superfamily member 19L | RELT | 0.87 | n.s | 0.91 | n.s |
| Other proteins | |||||
| Interleukin-15 receptor subunit alpha | IL15RA | 1.02 | n.s | 1.14 | n.s |
| Interleukin-17F | IL17F | 1.20 | n.s | 1.06 | n.s |
| Complement decay-accelerating factor | CD55 | 1.13 | n.s | 0.83 | n.s |
| CMRF35-like molecule 6 | CD300C | 1.01 | n.s | 0.92 | 2.6 × 10−3 |
| TNF ligand superfamily member 15 | TNFSF15 | 1.12 | n.s | 0.93 | n.s |
| C–C motif chemokine 14 | CCL14 | 0.61 | 8.8 × 10−5 | 0.15 | 1.9 × 10−5 |
| C–C motif chemokine 15 | CCL15 | 0.95 | n.s | 1.01 | n.s |
| Macrophage colony-stimulating factor 1 | CSF1 | 0.93 | n.s | 1.36 | n.s |
| Hepatitis A virus cellular receptor 2 | HAVCR2 | 1.03 | n.s | 0.97 | n.s |
| Interleukin-1 receptor type 1 | IL1R1 | 0.98 | n.s | 1.01 | n.s |
| Interleukin-18 receptor 1 | IL18R1 | 2.81 | 4.5 × 10−6 | 1.81 | 6.8 × 10−5 |
TNF, tumor necrosis factor; TNF-RSF, tumor necrosis factor receptor superfamily; n.s., not significant. Fold changes indicated in bold text indicate differentially expressed proteins at the Bonferroni’s correction α = 2.9 × 10−3 (Bonferroni’s correction for 17 KRIS proteins). Fold change is a ratio of a mean RFU concentration of a protein in HUVECs incubated with TNFα in hyperglycemia to a mean RFU concentration of a protein in HUVECs cultured in hyperglycemia.