Table 2.

List of 53 differentially expressed proteins and their corresponding intracellular and extracellular protein levels (expressed as fold changes) following TNFα treatment in hyperglycemia condition versus hyperglycemia alone.

Protein name	Gene symbol	Intracellular		Extracellular
		Fold change	p value	Fold change	p value
Significant intracellular and extracellular (n = 6)
C–C motif chemokine 5	CCL5	2.38	3.3 × 10−5	15.3	5.0 × 10−6
Cathepsin S	CTSS	2.82	2.7 × 10−5	2.58	1.8 × 10−5
Insulin-like growth factor-binding protein 4	IGFBP4	0.35	6.2 × 10−6	0.35	2.0 × 10−5
Inhibin beta A chain	INHBA	2.13	3.2 × 10−5	2.35	9.4 × 10−7
Protein jagged-1	JAG1	1.43	1.0 × 10−5	2.14	2.2 × 10−6
E-selectin	SELE	6.25	8.0 × 10−6	11.53	4.5 × 10−7
Significant intracellular (n = 7)
Complement C1q subcomponent	C1QA/B/C	1.11	3.7 × 10−5	1.06	n.s
Interleukin-8	CXCL8	9.44	2.8 × 10−5	1.11	n.s
Tyrosine-protein kinase Lyn	LYN	0.62	1.3 × 10−5	1.11	n.s
Tyrosine-protein kinase Lyn, isoform B	LYN	0.63	7.0 × 10−6	1.00	n.s
Mitogen-activated protein kinase 8	MAPK8	0.52	1.2 × 10−5	1.02	n.s
Nidogen-1	NID1	0.58	7.0 × 10−6	0.67	n.s
Neuropilin-1	NRP1	0.44	1.6 × 10−5	0.64	n.s
Significant extracellular (n = 40)
Angiopoietin-2	ANGPT2	0.64	n.s	0.27	1.1 × 10−5
Apolipoprotein E (isoform E4)	APOE	1.11	n.s	0.79	3.0 × 10−5
Carbonic anhydrase 4	CA4	1.16	n.s	1.11	2.1 × 10−5
Calcium/calmodulin-dependent protein kinase type II subunit delta	CAMK2D	0.68	n.s	1.35	2.3 × 10−5
C–C motif chemokine 20	CCL20	0.88	n.s	4.52	4.2 × 10−7
Complement factor I	CFI	0.89	n.s	0.64	8.1 × 10−6
Stem cell growth factor-beta	CLEC11A	1.09	n.s	0.66	9.5 × 10−6
Stem cell growth factor-alpha	CLEC11A	1.06	n.s	0.62	2.9 × 10−5
Collectin-12	COLEC12	0.66	n.s	0.58	5.1 × 10−6
Granulocyte colony-stimulating factor	CSF3	1.16	n.s	3.53	1.7 × 10−5
Cystatin-SA	CST2	1.11	n.s	0.8	5.1 × 10−6
Fractalkine	CX3CL1	1.17	n.s	2.69	2.0 × 10−5
Growth-regulated alpha protein	CXCL1	3.47	n.s	1.43	8.4 × 10−6
C-X-C motif chemokine 10	CXCL10	1.00	n.s	5.70	8.4 × 10−6
Gro-beta/gamma	CXCL3/L2	0.91	n.s	19.59	1.8 × 10−7
C-X-C motif chemokine 5	CXCL5	1.00	n.s	1.61	2.1 × 10−6
DnaJ homolog subfamily B member 1	DNAJB1	0.76	n.s	1.45	1.1 × 10−5
Ectodysplasin-A, secreted form	EDA	1.08	n.s	1.08	3.4 × 10−5
Fibroblast growth factor 18	FGF18	1.04	n.s	2.63	1.0 × 10−6
Heterogeneous nuclear ribonucleoprotein A/B	HNRNPAB	0.89	n.s	1.76	6.2 × 10−6
Intercellular adhesion molecule 1	ICAM1	3.97	n.s	2.96	1.2 × 10−5
Interleukin-37	IL37	1.16	n.s	1.11	1.7 × 10−5
Interleukin-6	IL6	2.02	n.s	4.25	1.9 × 10−5
Inosine-5′-monophosphate dehydrogenase 1	IMPDH1	0.73	n.s	1.65	4.1 × 10−6
Inhibin beta A:Inhibin beta B chain	INHBA/BB	1.22	n.s	3.56	3.7 × 10−7
Importin subunit alpha-1	KPNA2	0.64	n.s	1.26	1.0 × 10−5
Matrilin-2	MATN2	0.82	n.s	0.62	1.2 × 10−5
Stromelysin-2	MMP10	1.72	n.s	2.12	4.8 × 10−6
Glycylpeptide N-tetradecanoyltransferase 1	NMT1	0.93	n.s	1.55	7.0 × 10−6
Urokinase-type plasminogen activator	PLAU	1.00	n.s	4.74	5.6 × 10−6
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1	PLCG1	0.85	n.s	1.39	1.6 × 10−5
Plasma serine protease inhibitor	SERPINA5	1.12	n.s	0.81	9.9 × 10−6
Spondin-1	SPON1	0.97	n.s	0.76	3.2 × 10−5
Stanniocalcin-1	STC1	0.66	n.s	0.59	2.5 × 10−5
Metalloproteinase inhibitor 1	TIMP1	0.71	n.s	0.65	1.4 × 10−5
Tenascin	TNC	1.10	n.s	3.84	2.3 × 10−6
Tumor necrosis factor (internal control)	TNF	1.18	n.s	69.46	5.1 × 10−8
TNF-inducible gene 6 protein	TNFAIP6	1.01	n.s	1.69	7.2 × 10−6
Polyubiquitin K48-linked	UBB	0.82	n.s	1.55	1.6 × 10−5
X-ray repair cross-complementing protein 6	XRCC6	0.92	n.s	2.09	2.7 × 10−5

n.s., not significant. Threshold for the significance used: α = 3.8 × 10⁻⁵ (Bonferroni’s correction for 1305 proteins measured on the SOMAscan platform). Fold change is a ratio of a mean RFU concentration of a protein in HUVECs incubated with TNFα in hyperglycemia to a mean RFU concentration of a protein in HUVECs cultured in hyperglycemia alone condition.