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. 2021 May 27;4:632. doi: 10.1038/s42003-021-02166-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematics of the definition of anatomical regions based on multiplexed marker data are presented. Created in Biorender.com. b Morphological analysis of area ratios of DNA1, CD4, H3, CD68, Granzyme B, and E-cadherin markers in normal tonsil tissues is demonstrated. The scale bar is 200 µm. c Morphological coverage of the same subset of markers in diseased tonsil tissues is shown. d Bar plots for area ratios of Histone3, CD68, and Granzyme B markers in three normal tonsils (NT) and three diseased tonsils (DT) datasets are plotted. e Box plots of the mean area ratio of Histone3, CD68, and Granzyme B markers that were averaged from three sets of NT and DT data. Box plots show median first and third quartile, minimum, and maximum (excluding outliers).