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. 2021 May 14;15:672136. doi: 10.3389/fncel.2021.672136

FIGURE 3.

FIGURE 3

The expressions of BDNF mRNA in hippocampal astrocytes. (A) Primary hippocampal astrocytes were treated with 20 μM MK801 for 0, 24, or 48 h and BDNF mRNA expression was assessed by qPCR. Expression increased significantly after 24 h, but not after 48 h relative to untreated controls (0 h). (B) Two successive administrations of MK-801 for 24 h each also enhanced BDNF mRNA expression [(20 μM+20 μM)/48 h group] compared to untreated controls (Ctrl). (C) Primary hippocampal astrocytes were treated with 20 μM MK-801, 20 μM NMDA, or both for 24 h. mRNA levels were detected by qPCR. NMDA reduced basal BDNF mRNA expression and reversed the increase induced by MK-801. Expressed as the mean ± S.E.M of three independent experiments. Statistical differences between groups were determined by one-way ANOVA followed by Newman–Keuls multiple comparison tests. ∗P < 0.05 and ∗∗P < 0.01 vs. 0 h group or control group, #P < 0.05 and ##P < 0.01 vs. 24 h group or MK801 20 μM/48 h group.