Figure 6.

IL-1β production from PBMCs stimulated with ETVAX WCC or LPS ± dmLT. IL-1 β concentrations in cultures with PBMCs from (A, C) adults and (B, D) infants stimulated with increasing concentrations of ETVAX WCC (200-20,000 bacteria/ml) or ETEC O78 LPS (0.01-1 ng/ml) alone or with 10 µg/ml dmLT. Bars represent mean with SEM of IL-1β concentrations in culture supernatants (ETEC WCC 2000 bacteria/ml; adults n = 13, infants n = 14, 200 bacteria/ml; adults n = 9, infants n = 9, LPS 0.1 ng/ml; adults n = 12, infants n = 11, LPS 0.01 ng/ml; adults n=6, infants n=6). Statistical analysis was performed with the Wilcoxon matched-pairs signed rank test. ^* P < 0.05, ^** P < 0.01, ns, not significant; P > 0.05.