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. 2021 May 14;12:666584. doi: 10.3389/fmicb.2021.666584

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Copyright © 2021 Trego, McAteer, Nzeteu, Mahony, Abram, Ijaz and O’Flaherty.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A combined null-model approach to identify and quantify ecological community assembly processes between floating and settled granules using (A) environmental filtering calculated as nearest taxa index (NTI) where values > + 2 indicate extreme clustering in the phylogenetic tree driven by environmental pressures (determinism); (B) the stochasticity ratio approach, which quantified stochasticity using the Jaccard metric and Taxa-Richness constraints of proportional-proportional (P-P) and proportional-fixed (P-F) as a normalised stochasticity ratio (NST); and (C) the quantitative process estimates (QPE) approach which determines the proportion of assembly mechanisms acting on a category within the framework of selection, dispersal and undominated, represented by a stacked bar chart. Lines for (A) connect two categories where the differences were significant (ANOVA) with **(p < 0.01).