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. 2021 May 27;11:11237. doi: 10.1038/s41598-021-90881-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RNA-seq analysis of protein-coding gene expression in the muscle tissues of GF piglets and normal piglets. (A) Heatmap of 1156 significantly differentially expressed genes (DEGs). The red dots and the blue dots indicate up- or downregulated genes with fold changes > 2.0 and P adjusted < 0.05. (B) Abundance patterns of the genes segregated into two clusters after k-means analysis. (C) Top 10 upregulated genes and 10 downregulated genes in the muscle tissue of GF piglets vs. normal piglets. (D) DEGs enriched in the GO term muscle system process.