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. 2021 May 27;11:11153. doi: 10.1038/s41598-021-90600-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Splenectomy promoted adipocyte hypertrophy in mesenteric PVAT (mPVAT) of mice fed a high-fat diet (HFD). (a) Representative histological images, (b) mass, (c) adipocyte size, (d) HIF-1 α mRNA levels and (e) adiponectin mRNA levels in mPVAT from sham-operated (SHAM) or splenectomized (SPX) mice fed with standard diet (SD) or HFD. (n = 4 per group and n = 8 to 10 per group for PVAT mass). Data were expressed as mean ± SEM. ANOVA: *P < 0.05 and **P < 0.01, SD-SPX and HFD-SHAM versus SD-SHAM; ^$P < 0.05 and ^$$P < 0.01, HFD-SPX versus SD-SPX; ^##P < 0.01, HFD-SPX versus HFD-SHAM.