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. 2021 May 14;12:653571. doi: 10.3389/fimmu.2021.653571

Figure 5.

Figure 5

Requirement of mTORC1 for L-arginine/L-citrulline-enhanced glycolysis. PMΦs from C57Bl/6 (WT) or Nos2-/- mice were stimulated with Pam3Cys plus IFN-γ in R-free C-DMEM containing 400 μM L-arginine, L-citrulline, or neither amino acid with rapamycin or vehicle control (DMSO) for 24 hours. Following stimulation, cells were incubated in R-free Seahorse media containing 400 μM L-arginine (red), L-citrulline (blue), or neither amino acid (black) for 1 hour followed by ECAR analysis as in Figure 4 . Data are shown as (A) the differences in maximum ECAR between DMSO and rapamycin groups or (B, C) the raw ECAR values (N = 10, WT, two experiments combined; N = 5, Nos2 -/-, one experiment). **p < 0.01, ***p < 0.001, by 2-way ANOVA with Sidak’s post hoc test. Error bars, SD.