Table 1. Interpretation guide.
Procedure Subject |
A | B | C | D | Interpretation / Troubleshooting | |
Mesa formation | First patch test | Second patch test | Supernatantassay | Immunity assay | ||
Phage/host 1 | - | N/A | N/A | N/A | N/A | Phage unable to lysogenize host (?)Repeat A with lower phage titers and/or a longer incubation time |
Phage/host 2 | + | -* | N/A | N/A | N/A | Phage unable to lysogenize host |
Colony 1 | N/A | + | - | - | - | Phage carried-over from the streak plate, no lysogen production |
Colony 2 | N/A | + | + | + | + | Successful lysogen production |
Colony 3 | N/A | - | - | - | + | Phage resistance due to other mechanisms |
Colony 4 | N/A | + | - | - | + | False positive on the first patch test, phage resistance due to other mechanisms |
* At least 20 colonies screened, 10 from each of 2 different mesas. +: positive test. -: negative test.