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. 2021 Apr 20;11(8):e3997. doi: 10.21769/BioProtoc.3997

Table 1. Interpretation guide.

Most commonly encountered combinations of results and their interpretation.

Procedure

Subject

A B C D Interpretation / Troubleshooting
Mesa formation First patch test Second patch test Supernatantassay Immunity assay
Phage/host 1 - N/A N/A N/A N/A Phage unable to lysogenize host (?)Repeat A with lower phage titers and/or a longer incubation time
Phage/host 2 + -* N/A N/A N/A Phage unable to lysogenize host
Colony 1 N/A + - - - Phage carried-over from the streak plate, no lysogen production
Colony 2 N/A + + + + Successful lysogen production
Colony 3 N/A - - - + Phage resistance due to other mechanisms
Colony 4 N/A + - - + False positive on the first patch test, phage resistance due to other mechanisms

* At least 20 colonies screened, 10 from each of 2 different mesas. +: positive test. -: negative test.