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. 2021 May 19;13(5):755. doi: 10.3390/pharmaceutics13050755

Figure 5.

Figure 5

In vitro basic cytotoxicity screening of simple and 5-FU PEGylated SF NPs. The cytotoxicity of the pristine and 5-FU loaded SF/PEG NPs (12 mg/mL) was investigated after 24 h and 72 h of treatment in terms of: (A). Cell viability; (B). Mitochondrial integrity; (C). LDH release; (D). Cell viability and proliferation potential; (E). NO release (** p ≤ 0.01; **** p ≤ 0.0001). The fluorescence micrographs were obtained by after labelling HT–29 cells with Mitotracker and DAPI (B) or calceinAM and EthBr (D).