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. 2021 May 20;9(5):581. doi: 10.3390/biomedicines9050581

Figure 1.

Figure 1

Neurogenesis effect of AMA in PC12 cells. (a) Chemical structure of AMA. (b) Percentage of PC12 cells with neurite outgrowth after treatment with AMA at different doses or AMA combined with a low dose of the NGF. (c) Morphological changes in PC12 cells under an inverted optical microscope at 48 h after treatment with (i) control (0.5% DMSO); (ii) NGF (40 ng/mL); (iii) NGF (1 ng/mL); (iv) AMA (3 μM); (v) AMA (3 μM) + NGF (1 ng/mL). (d) Cell viability analysis results of PC12 cells after treatment with various doses of AMA or AMA combined with the NGF. Each experiment was repeated three times. The data were expressed as a mean ± SEM. *** indicates significant differences at p < 0.001 compared with the negative control and ### indicates a significant difference at p < 0.001 compared with the 3 μM AMA group.