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. 2021 May 20;26(10):3053. doi: 10.3390/molecules26103053

Figure 1.

Figure 1

Figure 1

Effects of Ba-ME on cell viability and inflammatory gene expression in LPS-treated cells. (a) Treated RAW264.7 cells with Ba-ME with the indicated concentrations for 24 h. Used MTT assay to measure the cell viability. The values are presented as mean ± SD of 3 replicates. (b) HEK293T cells were treated with different Ba-ME concentrations for 24 h, and the MTT assay was performed to determine cell viability. The values are presented as mean ± SD of 3 replicates. (c) Employing RT-PCR to detect the mRNA expression levels of CCL12, CXCL3, CXCL9, COX-2, and GAPDH in LPS-stimulated RAW264.7 cells treated with Ba-ME (0–50 μg/mL). Band intensity (the bottom panel of (c)) was measured and quantified using ImageJ. ** p < 0.01 compared with control cells.