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Effect of Ba-ME on intracellular signaling molecules’ activation in the AP-1 pathway. (a–c) Treated RAW264.7 cells with LPS (1 µg/mL) for 5, 15, 30, 60 min in the absence or presence of Ba-ME (150 µg/mL). A Western blot analysis determined the levels of phosphorylated forms and total forms of c-Jun and c-Fos (a). Using an immunoblot analysis of cell lysates to determine the phosphoprotein levels of p-38, ERK, JNK, and β-actin (b,c).
