Table 1.
Sequences of primers in this study.
| Primers | Sequence 5′-3′ (Forward/Reverse) | Reference |
|---|---|---|
| RT-qPCR | ||
| icaA | 5′-GATTATGTAATGTGCTTGGA-3′/ 5′-ACTACTGCTGCGTTAATAAT-3′ |
[6] |
| yycF | 5′-TGGCGAAAGAAGACATCA-3′/ 5′-AACCCGTTACAAATCCTG-3′ |
[6] |
| yycG | 5′-CGGGGCGTTCAAAAGACTTT-3′/ 5′-TCTGAACCTTTGAACACACGT-3′ |
[6] |
| sarA | 5′-AGATGGCCCTTCTTCAAATG-3′/ 5′-CCGCAATAATTCTTGTGACG-3′ |
This study |
| icaD | 5′-ATGGTCAAGCCCAGACAGAG-3′/ 5′-CGTGTTTTCAACATTTAATGCAA-3′ |
[6] |
| 16S rRNA | 5′-GTAGGTGGCAAGCGTTATCC-3′/ 5′-CGCACATCAGCGTCAACA-3′ |
[6] |
| AS yycF detection | ||
| First strand synthesis SAPCR |
5′-CGTATTATTAGATATCATGTTACCTGGTCG-3′ | This study |
| AS yycF detection SAAS |
5′-GTTCACGTGTCATTACTTGTCCCATATG-3′ | This study |
| 5′RACE | ||
| 5′RACE adapter | 5′-GCUGAUGGCGAUGAAUGAAC ACUGCGUUUGCUGGCUUUGAUGAAA-3′ |
First Choice RLM–RACE (RNA ligase-mediated rapid amplification of cDNA ends), Thermo Scientific |
| 5′ RACE outer primer (c) | 5′-GCTGATGGCGATGAATGAACACTG-3′ | First Choice RLM–RACE, Thermo Scientific |
| 5′ RACE inner primer (d) | 5′-CGCGGATCCGAACACTGCGTTTGCTG GCTTTGATG-3′ |
First Choice RLM–RACE, Thermo Scientific |
| Gene specific outer primer (a) | 5′-GGCGAAGATATTGAATTAACACATCGTG-3′ | This study |
| Gene specific inner primer (b) | 5′-CATATGGGACAAGTAATGACACGTGAAC-3′ | This study |