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. 2021 May 20;13(10):2486. doi: 10.3390/cancers13102486

Figure 4.

Figure 4

E4-induced ERα signaling in vitro. PGR mRNA expression normalized to TBP and GAPDH in MCF7 (A) and T47D cells (B) treated with vehicle (EtOH 0.01%), E2 (10−11M or 10−9M) or E4 (ranging from 10−12M to 10−5M) for 4 h. n = 3–4 independent experiments. One-way ANOVA analysis followed by Dunnett’s post-test. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001, * versus vehicle. (C) Representative Western Blot of PR (PRA and PRB) and ERα from MCF7 cells treated with vehicle (EtOH 0.01%), E2 (10−9M) or E4 (10−7M or 10−10M) for 24h. GAPDH was used as a loading control. (D) Quantification of PR expression normalized to GAPDH level, RI= Relative Intensity, n = 3 independent replicates. (E, F) Representative experiment of cell growth kinetics of MCF7 and T47D cells treated with vehicle (EtOH 0.01%), E2 (10−9M) or E4 (10−10M, 10−9M or 10−7M). (G, H) Proliferation rate after 72 h. Mann–Whitney test. *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001, * versus vehicle.