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Culture and characterization of cells. (A) Umbilical cord-derived mesenchymal stem cells (UC-MSCs) were cultured in Cytomix medium (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) and their adipogenic, osteogenic, and chondrogenic differentiation was studied using Oil Red O, Alkaline Phosphatase, and Alcian Blue, respectively. (B) Rabbit corneal epithelial cell line (SIRC) and (C) Human corneal epithelial (HCE) cells were characterized by expression of CK12. Bar size: 100 μm.
