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Validation of AS events and crucial gene expression. (a) The gene transcript structure and AS events of crucial genes in male chicken germ cell differentiation. The red square is exon, the different color cycles are AS events, and the purple arrow represents the qRT-PCR primers at the constitutive exon loci. (b) The gene expression of RNA-seq data. (c) The gene expression of qRT-PCR results and correlation analysis.
