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. 2021 May 20;7(5):399. doi: 10.3390/jof7050399

Figure 5.

Figure 5

Morphology of empty vector control strain (A) and brl overexpressing strains (B–F). Strain Brl1 OE (B) showed a straight growth but produced less dense aerial hyphae, while strain brl2OE (C) grows more irregularly, forming dense aerial hyphae. The mycelia of brl3 overexpressing strain Brl3–1OE (D) grows in a feathery manner with sectors growing faster than the rest of the mycelium, while Brl3–2OE (E) grows in a feathery manner but with dense aerial hyphae. Strain brl4OE (F) grows more irregularly when compared to the empty vector control strain forming dense aerial hyphae. Self-recognition of brl overexpressing strains (G–J). (G) Distance towards same clone mycelia was measured after 14 days from 5 biological replicates. (H) represents the empty vector control strain, while (I) shows the non-self-avoiding phenotype of the Brl1OE strain and the self-avoiding phenotype of Brl3OE (J) as an example. The Student’s t-test was used to determine the P value between control and transformants; (*) p < 0.005.