Domain or Specific Clinical Situations	Clinical, Laboratory, and Imaging Assessments or Measurement Instruments
CORE ASSESSMENTS
Severity assessment	UKMEB-PQsymp; DPQ, RAND-36, Pain and fatigue analogue scales
Disability screening	RAND-36 summary scales (physical and mental component summaries)
Muscle power and general health	Hand grip measurements, dynamometer
ADDITIONAL ASSESSMENTS
Routine tests not done recently and justified clinically	Tests as appropriate
If clinical history suggests autoimmune or immunodeficiency	ANA, ENA, TPO, AMA, APA, immunoglobulins, and others according to clinical findings
Serious neurocognitive symptoms that increase risks for patients	Neurocognitive tests—e.g., Creteil battery of tests; NIH CDE Toolbox (National Institute of Neurological Disorders and Stroke (NINDS), 2018) [43]
Neuroimaging as needed for further neurological investigations	MRI scan, CT
Obstructive sleep apnoea suspected	Sleep studies, polysomnography
Signs of small fibre neuropathy, peripheral neuropathy, marked muscle symptoms, objective peripheral findings	Nerve conduction studies, electromyography (EMG), skin (for intradermal nerve fibre density) or muscle (rarely necessary) biopsy
POTS, orthostatic intolerance	Tilt table test or repeated recumbent and standing heart rate and blood pressure (standing test)
Objective assessment of PEM or disability	2-day CPT (use with caution as can cause or aggravate PEM)
Other more recent tests which may be useful	Metabolomics, e.g., those revealed through organic acid testing and amino acid urine and serum, cytokine panels, and autoantibodies to receptors such as adrenergic receptors.
* A selection or the full range of tests may be conducted routinely or in support of disability assessment. AMA: anti-mitochondrial antibody. ANA: anti-nuclear antibodies. APA: anti-phospholipid antibodies. CPT: cardio-pulmonary testing. DPQ: DePaul Symptom Questionnaire. ENA: extractable nuclear antigens. PEM: post-exertional malaise. POTS: postural orthostatic tachycardia syndrome. TPO: thyroid peroxidase. UKMEB PQsym.: UK ME/CF Participant Questionnaire.