Figure 6.

Anti-biofilm effect of Octominin against A. baumannii. (A) Quantitative measurement of the biofilm formation inhibition by Octominin. A. baumannii was treated with increasing concentrations of Octominin (0–12.5 µg/mL) and chloramphenicol (10 µg/mL and 50 µg/mL). After 24 h of treatment, the remaining biofilm was stained with crystal violet (CV), and the OD was measured at 595 nm (OD₅₉₅); (B) Quantitative measurement of A. baumannii biofilm eradiation by Octominin. A. baumannii was allowed to form biofilm for 24 h and then the biofilm was treated with increasing concentrations of Octominin (0–12.5 µg/mL) and chloramphenicol (10 µg/mL and 50 µg/mL). After 24 h of treatment, the remaining biofilm was stained with CV, and the OD₅₉₅ was measured. * p < 0.05, compared to the control (0-Octominin) group. The error bar indicates the mean ± standard deviation (n = 3). PC = Positive control (chloramphenicol).