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SYBR real-time RT-PCR specific to the lyssavirus nucleoprotein. Standard curve and standards are shown in black. SYBR sample values (indicated by the red symbols) plotted and interpolated using the standard curve regression line (Y = −3.607 × LOG(X) + 40.76) (R2 = 0.995; efficiency = 91%) and the mean Ct values. Triangles represent cSN-KBLV-mock-vaccinated mice and squares represent cSN-KBLV-vaccinated mice.
