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. 2021 May 19;11(5):759. doi: 10.3390/biom11050759

Figure 4.

Figure 4

PDLIM5 knockdown attenuates the osteogenic differentiation of HSF mediated by microfilaments. HSFs treated with osteogenic differentiation medium (OS) with or without knock-down of PDLIM5 at 7 days. β-actin and osteogenesis-associated gene expression by (A) RT-qPCR and (B) Western blotting analyses. Densitometric quantification of the Western blotting bands normalized to GAPDH. * p < 0.05, ** p < 0.01, ***p < 0.001, n = 3. ALP staining (C) at 14 days. Scale bar = 100 µm. The effect of knocking down PDLIM5 on the morphology of microfilament skeleton was detected by immunofluorescence (D), a: knockdown PDLIM5 in GM (undifferentiated) group; b: knockdown PDLIM5 in osteogenic medium group for 7 days, Scale bar = 10 µm. (E) The effect of JAS on the osteogenic differentiation of HSF after knocking down PDLIM5. Densitometric quantification of the Western blot normalized to GAPDH. * p < 0.05, ** p < 0.01, *** p < 0.001, n = 3. ALP staining (F) at 14 days of HSFs osteogenic medium with JAS after knocking down PDLIM5 expression. Scale bar = 100 µm.