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. 2021 May 19;11(5):329. doi: 10.3390/metabo11050329

Figure 6.

Figure 6

SLC7A11 mRNA expression correlates with the amount of iron-loaded Lcn-2 in tumor tissue of ccRCC patients. (A) mRNA expression of SLC7A11 in samples of tumor tissue of ccRCC patients (n = 32) compared to samples of adjacent healthy tissue of the same patients. (B,C) SLC7A11 mRNA expression in healthy tissue, (B) G1–G2 (n = 23) and G3–G4 (n = 9) tumor grade and (C) pT1–pT2 (n = 18) and pT3–pT4 (n = 14) tumor stage. mRNA expression was normalized to housekeeping gene 18S expression. (D) The amount of hLcn-2 was analyzed by AAS measurements in Lcn-2-immunoprecipitated samples of tumor tissue of ccRCC patients (n = 25) and compared to samples of adjacent healthy tissue of the same patients. (E,F) Lcn-2-bound iron in healthy tissue, (E) G1–G2 and G3–G4 tumor grade, and (F) pT1–pT2 and pT3–pT4 tumor stage (n = 25). (G) Correlation analysis between SLC7A11 mRNA expression and the amount of AAS-detected hLcn-2 in tumor tissue of ccRCC patients (n = 25). Significance of the correlation was determined by Spearman’s test including all investigated groups. Graphs are displayed as (A–F) violin plots, indicating the median and quartiles with whiskers reaching up to 1.5 times the interquartile range and (G) simple linear regression of correlations, with * p < 0.05, ** p < 0.01, *** p < 0.001.