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. 2021 May 19;18(10):5436. doi: 10.3390/ijerph18105436

Table 3.

Positive samples with low and high burden of accompanying microbial flora from qPCR and culture for L. pneumophila and L. pneumophila sg 1, established from the L. pneumophila qPCR for the mip gene and the L. pneumophila sg 1 qPCR for the wzm gene.

Comparison of qPCR and Culture
low burden of accompanying microbial flora (n = 46)
No. of positive samples culture qPCR
Legionella pneumophila (mip) 9 4
(8 culture positive–qPCR negative) (3 qPCR positive–culture negative)
Legionella pneumophila sg1 (wzm) 0 0
high burden of accompanying microbial flora (n = 18)
No. of positive samples culture qPCR
Legionella pneumophila (mip) 2 5
(3 qPCR positive–culture negative)
Legionella pneumophila sg1 (wzm) 1 5
(4 qPCR positive–culture negative)