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Aging contribution to changes of ZO-1 density. Comparison between RPEs of wt animals, aged 12 (n = 3) and 20 (n = 6) months, respectively, distinguishing central (cen RPE) and peripheral (per RPE). Two-way ANOVA p = 0.0162. Post hoc Sidak’s test. wt 12 m cen RPE vs. wt 20 m cen RPE, p = 0.0095; wt 12 m per RPE vs. wt 20 m per RPE, p > 0.999. Error bars represent ±SEM. ** p < 0.01; ns: not significant.
