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Reactivity-based screen of putative tetraene-containing GPMs. (a) Methanol-extracted metabolites from S. natalensis (natamycin producer) and S. viridosporus show robust labeling after reaction with probe 1, while no labeling was observed for A. spheciospongiae. Mass of the parental GPM (unlabeled, top) is denoted with an asterisk, while the labeled GPM is indicated in red (bottom). (b) Structures of tetraene-containing GPMs with key carbons of kineosporicin numbered.
