Figure 3.
In vitro serum stability, cellular uptake, and immune activation by L-SNAs. (a) Plot of the initial rate of decay, k, as a function of decrease in FRET signal over time. Changing the liposome scaffold from DOPC to one comprising phospholipids of higher TC significantly decreases the rate of DNA dissociation from L-SNAs, thus increasing the stability of the overall construct. (b) Cellular uptake of L-SNAs by DCs as a function of liposome scaffold. Uptake is significantly increased by synthesizing L-SNAs from all higher-TC lipids. (c) DC activation as a function of L-SNA composition. Changing the liposome scaffold from DOPC to one comprising phospholipids of higher TC significantly increases the observed expression of CD86. Statistical analysis was performed using an unpaired t test, where “**” represents a p value of <0.01, “***” represents a p value of <0.001, and “****” represents a p value of <0.0001. Error bars represent standard deviations. MFI represents median fluorescence intensity.