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. 2021 May 14;12:646736. doi: 10.3389/fpls.2021.646736

Figure 1.

Figure 1

PIP2 is an auxin response gene. (A) Expression of PAMP-INDUCED SECRETED PEPTIDES (PIPs) in response to auxin treatment. Ten-days-old Col wild type seedlings were treated with 10 μM INDOLE-3-ACETIC ACID (IAA) for 4 h, total RNA was isolated and quantitative RT-PCR (qRT-PCR) was used to examine the expression of PIPs. Expression of ACTIN2 (ACT2) was used as an inner control, and the expression level of corresponding PIP genes in the control seedlings was set as 1. Data represent mean ± SD of three repeats. *significantly different from absent of IAA (student’s t test, p < 0.001). Expression of PIP2 (B) and IAA19 (C) in the nph4-1 and arf19-4 mutants in response to auxin treatment. Ten-day-old Col wild type, nph4-1 and arf19-4 mutant seedlings were treated with 10 μM IAA for 4 h. Total RNA was isolated and qRT-PCR was used to examine the expression of PIP2 or IAA19. Expression of ACT2 was used as an inner control, and the expression level of PIP2 or IAA19 in control seedlings of the Col wild type was set as 1. Data represent mean ± SD of three repeats. *significantly different from the corresponding expression level in the Col wild type seedlings (student’s t-test, p < 0.001). The experiments were repeated three times with similar results.