Figure 3.

Generation of the pip2, pip3, and pip2 pip3 mutants by CRISPR/Cas9 gene editing. (A) Editing status of PIP2 in the pip2 and pip2 pip3 mutants. (B) Editing status of PIP3 in the pip3 and pip2 pip3 mutants. DNA was isolated from leaves collected from early bolting T1 plants or normal bolting T2 plants, and PCR was used to amplify the coding sequence of PIP2 and/or PIP3. The PCR products were recovered and sequenced, and sequencing results were compared with genome sequence of PIP2 or PIP3 to check the editing status. Dash lines indicate the target sequences, and solid lines indicate the PAM sites.