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Amino acid alignment of PIP2 and PIP3 in the Col wild type and the pip2, pip3 and pip2 pip3 mutants. The open-reading frame (ORF) of PIP2 and PIP3 sequences in the pip2 and pip2 pip3 mutants were identified by using ORFfinder (https://www.ncbi.nlm.nih.gov/orffinder/), and corresponding amino acid sequences were used for alignment with the amino acid sequences of PIP2 and PIP3, respectively. Under lines indicate the mature PIP2 and PIP3 peptides.
