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. 2021 May 14;12:646736. doi: 10.3389/fpls.2021.646736

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Copyright © 2021 Hussain, Wang, Ahmed, Wang, Adnan, Cheng, Wang, Wang, Zhang, Tian, Chen, Hu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PIP2 and PIP3 affect root elongation. (A) Root elongation in seedlings of the Col wild type, the 35S:PIP2 and 35S:PIP3 transgenic plants, and the pip2, pip3 and pip2 pip3 mutants. Seeds were sterilized and plated on 1/2 Murashige and Skoog (MS) plates, kept at 4°C and in darkness for 2 days before transferred to a growth room and grown vertically for 8 days. Pictures were taken by using a digital camera. (B) Root length in seedlings of the Col wild type, the 35S:PIP2 and 35S:PIP3 transgenic plants, and the pip2, pip3, and pip2 pip3 mutants. Root length of 8-day-old seedlings was measured. Data represent the mean ± SD of 21–25 seedlings. *significantly different from that of the Col wild type seedlings (student’s t-test, p < 0.001). The experiments were repeated three times with similar results.