Figure 7.

PIP2 affects cell prolification and cell elongation in hypocotyls. (A) Hypocotyl cells in seedlings of the Col wild type, the 35S:PIP2 and 35S:PIP3 transgenic plants, and the pip2, pip3, and pip2 pip3 mutants. Four-day-old dim light-grown seedlings were fixed and pictures were taken under an OLYMPUS BX53 microscope. (B) Number of hypocotyl cell in seedlings of the Col wild type, the 35S:PIP2 and 35S:PIP3 transgenic plants, and the pip2, pip3 and pip2 pip3 mutants. Cell number of a single cell line of the 4-day-old seedlings grown under dim light was counted under an OLYMPUS BX53 microscope. Data represent the mean ± SD of 10–12 seedlings. *significantly different from that of the Col wild type seedlings (student’s t-test, p < 0.001). The experiments were repeated three times with similar results. (C) Hypocotyl cell length in seedlings of the Col wild type, the 35S:PIP2 and 35S:PIP3 transgenic plants, and the pip2, pip3, and pip2 pip3 mutants. Length of the second row cells from the top to the base of the hypocotyls epidermis in longitudinal direction were was measured by using Image J. Data represent the mean ± SD of 22–29 seedlings. *significantly different from that of the Col wild type seedlings (student’s t-test, p < 0.001). The experiments were repeated three times with similar results.