FIGURE 5.

Lack of miR‐146a/b in mice results in an altered immune response in allergic airway inflammation and RV‐induced exacerbation of allergic airway inflammation models. (A) Mice were sensitized (D0) and challenged (D7‐11) with house dust mite extract (HDM) and, when indicated, infected with RV‐A1b (D21) for 24 h. Control mice received only PBS. (B) BAL cells were counted with a hemocytometer and then subjected to FACS analysis. From the dot plot percentages, the total numbers of immune cells were calculated. Data represent mean ± SEM. One‐way ANOVA with Tukey's multiple comparisons test and adjusted p‐values are shown. + p < 0.05, ++ p < 0.01 HDM compared to the same mouse line with PBS; ## p < 0.01 HDM+RV‐A1b compared to same mouse line with HDM; *p < 0.05, **p < 0.01, ***p < 0.001 wt compared to Mir146a/b^−/− mice for the same treatment. (C) One representative FACS dot plot of BAL fluid from five HDM‐treated or HDM+RV‐A1b‐treated wt and Mir146a/b^−/− mice. Data from one representative of three independent experiments are shown