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HPAC-PAD profiles of overnight reactions with 0.1% PASC and 50 µl of the fivefold unpurified, concentrated expression supernatant containing NcAA9C expressed with different signal peptides in the presence (solid lines) and absence (dashed lines) of 1 mM AscA. The chromatograms in blue show the NcAA9C expressed with the native signal peptide. The OST1-produced LPMO is shown in red and the reaction using the enzyme secreted with the pre-OST1-pro-α-factor is presented in green
