Table 1.

Overview on the protein concentration (mg/ml) and total amount of protein (mg) of each step along the purification process of LsAA9A and NcAA9C expressed with either the pBSY3Z or the pBSY_PGCW14Z expression plasmid and the native LPMO SP, from the crude supernatant to the pure protein

Enzyme	Plasmid + SP	Supernatant		Fivefold concentrated		Purified (obtained from 50 ml cultivation supernatant)		Recovered protein
		mg	mg/ml	mg	mg/ml	mg	mg/ml	%
LsAA9A	pBSY3Z + native	21 ± 5	0.042 ± 0.010	22.5 ± 0.4	0.225 ± 0.004	0.61 ± 0.01	1.2 ± 0.02	27
	pBSYPGCW14Z + native	28 ± 2	0.056 ± 0.004	24.7 ± 1.7	0.247 ± 0.017	0.6 ± 0.03	1.4 ± 0.05	24
NcAA9C	pBSY3Z + native	22.5 ± 1.5	0.045 ± 0.003	20.7 ± 2.3	0.207 ± 0.023	0.39 ± 0.02	0.8 ± 0.05	18
	pBSYPGCW14Z + native	76 ± 7	0.153 ± 0.014	70.5 ± 0.5	0.705 ± 0.005	2.08 ± 0.06	2.9 ± 0.03	29

For precise measurements, the volume of the supernatant and the fivefold concentrate was adjusted to 500 ml and 100 ml, respectively. Please note that the values reported in the column labeled with “purified” were obtained from 50 ml cultivation supernatant