Table 2.

Comparison of expression constructs, cultivation and purification methods and the thereof resulting yields of different LPMO production protocols found in literature

Enzyme	Plasmid	Promoter	Signal peptide	Cultivation method	Purification steps	Total protein per liter culture media prior purification	Total protein per liter culture media after purification	Reference
TrCel61A	pPpT4	AOX1	Native	2-L fed-batch reactor	N.A	> 400 mg	N.A	[18]
NcAA9J	pPICZαA	AOX1	Native	7-L fed-batch reactor	Three chromatographic steps	1574 mga	24 mga	[22]
NcAA9C	pPICZαA	AOX1	Native	7-L fed-batch reactor	Two chromatographic steps	2762 mg	67 mga	[22]
NcAA9F	pPICZαA	AOX1	Native	7-L fed-batch reactor	Three chromatographic steps	1818 mga	2.4 mga	[22]
NcAA9E	pPICZαA	AOX1	Native	7-L fed-batch reactor	Three chromatographic steps	1327 mga	2.2 mga	[22]
NcAA9C	pPICZαA	AOX1	Native	500-mL fed-batch reactor	Two chromatographic steps	1370 mga	90 mga	[29]
AaAA16	pPICZαA	AOX1	Native	1.3-L fed-batch reactor	IMACb, IEC	N.A	500 mg after IMAC	[30]
Several AA9s	pPICZT	AOX1	Native	2-L shake flask	IMAC	34 mg per 100 g cell wet weight	N.A	[31]

^aYield was adjusted to 1 L culture supernatant

^bResulted in LPMO inactivation