Table 5.
List with the theoretical peptide masses of the trypsin digested NcAA9C expressed with the different signal peptides
| Fragment | Masses expected if the signal peptide is cleaved correctly | Masses expected of a wrongly processed N-terminus | ||
|---|---|---|---|---|
| Independent of SP | Native | OST1 | pre- OST1-pro-α-factor | |
| 1 | 773.4304 | 1986.1018 | 3104.5855 | 6754.3264 |
| 2 | 1215.6328 | 1215.6328 | 1215.6328 | 890.4465 |
| 3 | 2505.1782 | 2505.1782 | 2505.1782 | 1173.5898 |
| 4 | 1171.6317 | 1171.6317 | 1171.6317 | 1215.6328 |
| 5 | 2725.2865 | 2725.2865 | 2725.2865 | 2505.1782 |
| 6 | 991.5645 | 991.5645 | 991.5645 | 1171.6317 |
| 7 | 1234.6273 | 1234.6273 | 1234.6273 | 2725.2865 |
| 8 | 945.4999 | 945.4999 | 945.4999 | 991.5645 |
| 9 | 3347.5322 | 3347.5322 | 3347.5322 | 1234.6273 |
| 10 | 5926.898 | 5926.898 | 5926.898 | 945.4999 |
| 11 | 6678.2651 | 6678.2651 | 6678.2651 | 3347.5322 |
| 12 | 1020.5142 | 1020.5142 | 1020.5142 | 5926.898 |
| 13 | 1469.7091 | 1469.7091 | 1469.7091 | 6678.2651 |
| 14 | 1038.5037 | 1038.5037 | 1038.5037 | 1020.5142 |
| 15 | 1355.5718 | 1355.5718 | 1355.5718 | 1469.7091 |
| 16 | 1359.5674 | 1359.5674 | 1359.5674 | 1038.5037 |
| 17 | 1355.5718 | |||
| 18 | 1359.5674 | |||
The second column shows the expected masses of a correctly processed enzyme and is therefore independent of the leading sequence. In the columns 3 to 5 the expected masses of wrongly processed N-termini of the different signal peptides are listed. Highlighted in bold are the masses of the peptides containing the N-terminal histidine