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. 2021 May 28;26:22. doi: 10.1186/s11658-021-00269-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Hypoxia influenced the chondrogenic differentiation of rMSCs. (A) Isolated rMSCs were identified by flow cytometry. B The rMSCs were cultured with MCDM under hypoxic conditions (1, 2, 4, 4 8, 16, 21% O₂) for 14 days. Proteoglycan deposition was shown by Alcian blue staining assays. C Cell activity was showed by Cell Counting Kit-8. *p < 0.05