Fig. 4.

Effect of direct transcriptional regulation of miR-29a on MDM2 expression. A A typical representation of the putative binding site of miR-29a in the 3ʹ-UTR of MDM2. B, C Firstly, the wild-type or mutated full-length 3ʹ-UTR of the MDM2 gene was cloned into dual-luciferase reporter vectors; secondly, the vectors were co-transfected with miR-29a mimics, inhibitors or corresponding controls; finally, the activity was evaluated by luciferase reporter assay. D In glioma cells with miR-29a mimic, inhibitor or corresponding control transfection, relative mRNA levels of MDM2 were measured by relative quantity RT-PCR. E–F In glioma cells mentioned above, protein levels of MDM2 were detected by western blotting. Results are expressed as the mean ± SD (n = 3). *P < 0.05; **P < 0.01