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The metalloproteomics pipeline, where cells are lysed under native conditions (no detergent employed), and subjected to two dimensions of chromatography: anion exchange chromatography and size exclusion chromatography. The pipeline separates a single biological sample into 384 native samples suitable for protein, metal and enzyme assay analyses. Comparison of protein and metal distributions can elucidate major metal metalloprotein reservoirs within the soluble cellular lysate.
