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. Author manuscript; available in PMC: 2021 May 28.
Published in final edited form as: J Am Soc Mass Spectrom. 2020 Jul 24;31(12):2426–2436. doi: 10.1021/jasms.0c00119

Figure 3.

Figure 3.

MALDI IMS signals consistent with localization to photoreceptor and RPE compartments. (A) Schematic diagram of outer retina and Bruch’s membrane, excerpted from Figure 2A. Blue, pink, yellow, and green bands indicate layers formed by highly compartmentalized and vertically aligned photoreceptors and RPE cells in panels B and C. See Figure 2 for explanation of cellular and subcellular content of each layer. Layers: OPL, outer plexiform layer; ONL, outer nuclear layer; ELM, external limiting membrane; RPE, retinal pigment epithelium; BrM, Bruch’s membrane; R, Rod; C, cone photoreceptors. (B–F) Images and H&E stained tissue images overlaid in peripheral retina displaying signals from multiple lipid classes that localize to subcellular compartments of the photoreceptor cells. (B) Overlay showing four separate signals defined in panels C–F. (C) Localized to ONL. (D) Localized to photoreceptor inner and outer segments. (E) Localized to mitochondria-rich photoreceptor inner segments. (F) Localized to RPE apical processes.